Purification and Partial Characterization of Tomato Extensin Peroxidase
نویسندگان
چکیده
منابع مشابه
Purification and Partial Characterization of Tomato Extensin Peroxidase.
Early plant defense response is characterized by elevation of activity of peroxidases and enhanced insolubilization of hydroxyproline-rich glycoproteins, such as extensin, in the cell wall. The insolubilization process (cross-linking between soluble extensin precursor molecules) is catalyzed by extensin peroxidases. We have ionically eluted extensin peroxidases from intact water-washed suspensi...
متن کاملPURIFICATION AND SOME PARTIAL CHARACTERIZATION OF PEROXIDASE ISOENZYME FROM BRASSICA OLERACEA CAPITATA L.
Acetone fractionated peroxidase from crude extract of Brassica oleracea leaves (Cabbage) was purified in three steps on chromatographic columns, using Sp-Sepharose, DEAE-Sepharose and Con A-Sepharose. The specific activity of purified main isoenzyme (BOC-POD) is 1887 u/mg protein with RZ: 3.1, which is 172 times more than the RZ of crude extract with 4.3% recovery. The molecular weight of BOC-P...
متن کاملPartial purification of tomato fruit peroxidase and its effect on the mechanical properties of tomato fruit skin.
Peroxidase (EC 1.11.1.7)-mediated stiffening of cell walls within the fruit skin of tomato is hypothesized to regulate fruit growth. However, to date, there is no experimental evidence demonstrating that peroxidase affects the mechanical properties of skin tissue. Here, the mechanical properties of skin strips excised from a range of fruits at different ages were determined using an 'Instron' u...
متن کاملAn investigation of tomato extensin.
Plant dcfcncr rcsponscs to pathogcn clicitation, plant ccll growth and dcvclopmcnt arc complex and highly integrated pmcesses. Intcraction betwecn host plant and pathogcn result in significant differences in defence gcnc cxprcssion. A mynad 01 defence-related gcncs arc induccd in many caws of inumpati blc interaction betwccn host plant and pathogcn. Tcchniqucs that monitor individual rcsponscs ...
متن کاملpurification and some partial characterization of peroxidase isoenzyme from brassica oleracea capitata l.
acetone fractionated peroxidase from crude extract of brassica oleracea leaves (cabbage) was purified in three steps on chromatographic columns, using sp-sepharose, deae-sepharose and con a-sepharose. the specific activity of purified main isoenzyme (boc-pod) is 1887 u/mg protein with rz: 3.1, which is 172 times more than the rz of crude extract with 4.3% recovery. the molecular weight of boc-p...
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ژورنال
عنوان ژورنال: Plant Physiology
سال: 1995
ISSN: 0032-0889,1532-2548
DOI: 10.1104/pp.109.3.1115